Biochemical characterization of rice glutelin
WebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/α-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable … WebSep 1, 2024 · Here we investigated and characterized the formation of thermal-induced rice glutelin fibrils at 85 °C and pH 2.0. The potential effects of rice glutelin fibrils on the in vitro starch digestibility were also evaluated. Thioflavin T fluorescence spectral analysis revealed extensive formation of rice glutelin fibrils after 4 h of incubation at pH 2.0 and 85 °C.
Biochemical characterization of rice glutelin
Did you know?
WebJun 15, 2009 · In this study, transgenic a123 expressing glutelin with a mutation at the processing site proved to be useful for studying the glutelin sorting and assembly system in rice seeds. The results obtained using transgenic rice plants clearly address the functional role of post-translational processing from the glutelin precursor to the mature acidic ... WebJul 5, 2024 · Background Rice is not only an essential food but also a source of high quality protein. Polyploidy is an evolutionary trajectory in plants, and enhancing glutelin by polyploidization is an attractive strategy for improving the nutritional value of rice seeds and presents a great potential for enhancing the commercial value of rice. Elucidating the …
WebLikeoatglobulin (1), rice glutelin appears to be preferentially synthesized on membrane-bound polysomes (15, 29) and it is packaged in protein bodies (29). In addition, recent data indicates that ... WebUnlike other cereals which accumulate prolamins as their primary nitrogen reserve, the major class of storage proteins in rice and oat are the glutelins and 12S globulins, respectively. These proteins are highly homologous at the primary sequence level to the leguminous US globulins. This similarity in protein structure extends to the molecular ...
WebJun 26, 2007 · The overall electrophoretic profile and alpha glutelin subunits in different rice samples ranged from 12.1 to 100.9 and 32.1 to 34.0 kDa, respectively, while beta glutelin subunits ranged from 23. ... WebNov 2, 2008 · Abstract. A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the …
WebBiochemical Characterization of Rice Glutelin1 Received for publication September 25, 1984 and in revised form January 14, 1985 TUAN-NAN WEN AND DAWN S. LUTHE* …
WebDec 31, 2024 · Enhancing the solubility of rice glutelin in neutral aqueous solution is the prerequisite for the development of rice protein drinks and ingredients. Herein, glutelin … dutch style sofaWebEurope PMC is an archive of life sciences journal literature. dutch style shopWebREP-1 was further purified to a single polypeptide of 36 kDa. REP-1 digested in vitro both the acidic and basic subunits of rice glutelin, the major seed storage protein of rice. Determination of the N-terminal amino acid sequence and experiments with protease inhibitors indicated that REP-1 is a cysteine endopeptidase. in a flask of volume v litresWebMay 1, 1985 · The two major subunits of rice glutelin, the acidic (α) and basic (β) polypeptides were purified by chromatofocusing and cation exchange … in a flashy and tasteless wayWebJan 1, 1992 · The 51-kDa protein is the rice glutelin precursor, which is processed to give rise to the 34-37-kDa u-subunit and 21-23-kDa subunit [3,7]. Similarly, the 14-kDa protein has been identified as a rice prolamine [7]. The 25- and 16-kDa proteins were the most abundant polypeptides in the globulin fraction (Fig. 1, lane C). ... Biochemical ... dutch subtitles babylonWebChickpeas are the third most abundant legume crop worldwide, having a high protein content (14.9–24.6%) with interesting technological properties, thus representing a sustainable alternative to animal proteins. In this study, the surface and structural properties of total (TE) and sequential (ALB, GLO, and GLU) protein fractions isolated from defatted … in a flat toneWebNov 2, 2008 · Rice glutelin originates from the same ancestral gene as 11S globulin. Rice glutelin is synthesized as a M r 57 000 (57 kDa) precursor protein and is transported … dutch style soccer