Myc shrna

Author: dhxu

August undefined, 2024

Web1 jan. 2015 · MYC is aberrantly expressed in a variety of different forms of cancer and plays an important role in the biology of various lymphoma subtypes such as Burkitt lymphoma … WebMouse Myc shRNA Lentivirus, LSV-0049. Human MYC shRNA Lentivirus, LSV-0048. Mouse Myc ORF cDNA Lentivirus, LCV-0002. Recommended Controls. Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).

The splicing factor RBM25 controls MYC activity in acute …

WebHere we report that c-Myc is highly expressed in glioma cancer stem cells relative to non-stem glioma cells. To interrogate the significance of c-Myc expression in glioma cancer … Web12 jun. 2024 · The sEVs of Myc-manipulated SCLC cells were isolated using total exosome isolation reagents (from cells) (Life Technologies, USA). The sEVs were harvested from … tanner hall southern miss baseball

SANTA CRUZ BIOTECHNOLOGY, INC. c-Myc (C-8): sc-41 - SCBT

WebshRNA sequences correspond to N-Myc siRNA Gene Silencer sequences; provided as transfection-ready purified plasmid DNA target-specific gene silencing; after transfection, … WebIn HCT-116 and HT-29 cell, we adopted shRNA to silence c-myc expression and examined the effects. As shown in Figure 4A – B , in c-Myc knockdown cells, the expression of hexokinase-2 was dramatically decreased, the glucose consumption and lactate production were significantly dropped accordingly. WebCatalog number: SH821514. Description: Four pre-designed shRNA constructs targeting v-myc myelocytomatosis viral oncogene homolog (avian) (MYC) and one scrambled … tanner health system healthstream

N-Myc siRNA (h), shRNA and Lentiviral Particle Gene Silencers

Repression of PCGF1 Decreases the Proliferation of Glioblastoma …

WebMyc shRNA Alt name Myc Species H. sapiens (human) Insert Size (bp) 64 Entrez Gene MYC ( a.k.a. MRTL, MYCC, bHLHe39, c-Myc) Cloning Information Cloning method … WebU2OS MYC/ER cells were infected with lentiviral POLRMT shRNA or Luciferase (Luc) shRNA as a control. Five days post-infection cells were seeded at equal densities and … tanner hall jefferson county tnWeb13 apr. 2024 · Silencing TMEM40 with shRNA inhibited the invasion, migration and proliferation, promoted apoptosis and led to a G0/G1 cell cycle arrest in CC cell lines. Silence of TMEM40 downregulated the expression of c-MYC, Cyclin D1, matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9), but in contrast, … tanner health

"WebLentiviral plasmid encoding shRNA targeting AKR1C3 (TRCN0000026561) ... Here our data showed that c-Myc is also a downstream target of AR-V7 which suggests a possible reciprocal regulation of AR-V7 and c-Myc. Knockdown of AKR1C3 suppresses both AR-V7 and c-Myc expression in resistant cells. " - Myc shrna

Myc shrna

PLK1 contributes to autophagy by regulating MYC stabilization in ...

WebFigure 1 Knockdown of PCGF1 by lentivirus-mediated short hairpin RNA (shRNA)-induced cell cycle arrested at G0/G1 phase and attenuation of proliferation in U87 cells.Notes: (A) Expression analysis of PCGF1 mRNA by RT-qPCR analysis.(B) Expression of PCGF1 proteins in infected cells was analyzed by Western blot.(C) Cell cycle distribution of U87 …

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Weband c-Myc shRNA (m) Lentiviral Particles: sc-29227-V. c-Myc (N-262) X TransCruz antibody is recommended for Gel Supershift and ChIP applications. Molecular Weight of c-Myc: 67 kDa. STORAGE Store at 4° C, **DO NOT FREEZE**. Stabl efor one year from the date of shipment. Non-hazardous. Web10 aug. 2024 · shRNA Plasmid. shRNA Lentiviral Particles. Description. 10 µM, 50-100 transfections. siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression. for independent verification of Myc gene silencing results, individual siRNA duplex components are also available upon …

WebUpon transient transfection with plasmid-encoding shRNA, it was found that expression of c-Myc decreased in shRNA-transfected cells, and the downregulation of c-Myc inhibited cell growth and induced apoptosis in Colo 320 cells. c-Myc downregulation also increased cell population in the G0-G1 phase. In conclusion, our findings demonstrate that ... WebshRNA プラスミドは、ノックアウト最大化のために設計される3から5種類のターゲット特異的な19-25ntのレンチウイルスベクタープラスミドのプールから構成されます。 shRNA配列 N-Myc siRNA Gene Silencer sequences

Web20 sep. 2013 · YAP or c-Myc-specific shRNAs with high knockdown efficiency ( Fig. 1A and B) were used to silence protein expression in both Bel-7402 and SMMC-7721 cells. We found that inhibition of either YAP or c-Myc decreased cell proliferation compared to control, as measured by a MTT-based assay (Fig. 1 C). Web12 okt. 2024 · MYC proto-oncogene family including c-myc and n-myc (MYCN) are critical for normal cell development and tumorigenesis. Overexpression of c-myc causes acute …

Web11 apr. 2024 · STAT3 inhibition attenuates MYC expression by affecting recruitment of histone acetyltransferase p300, thereby reducing enrichment of H3K27 acetylation in the MYC promoter. Concomitantly, it also decreases the occupancy of the bromodomain containing ... using inducible STAT3 shRNA and a clinically relevant STAT3 inhibitor …

Web12 jun. 2024 · The sEVs of Myc-manipulated SCLC cells were isolated using total exosome isolation reagents (from cells) (Life Technologies, USA). The sEVs were harvested from H82 cont-shRNA, H82 Myc-shRNA, H209 Mock, and H209MYC cells cultured in RPMI-1640 medium supplemented with 10% exosome-depleted FBS in 25 cm 2 flasks and 6-well … tanner health source gymWeb15 aug. 2014 · Pharmacological or shRNA-mediated CDK9 inhibition led to robust anti-tumor effects that correlated with MYC expression levels and depended on the role that … tanner health roanoke alWebParticles: sc-29226-V, c-Myc shRNA (m) Lentiviral Particles: sc-29227-V and c-Myc shRNA (r) Lentiviral Particles: sc-270149-V. c-Myc (C-8) X TransCruz antibody is recommended for ChIP assays. Molecular Weight of c-Myc: 67 kDa. Positive Controls: c-Myc (m): 293T Lysate: sc-118892, c-Myc (h): 293T Lysate: tanner health system hrWebMyc shRNA Alt name Myc Species H. sapiens (human) Insert Size (bp) 64 Entrez Gene MYC ( a.k.a. MRTL, MYCC, bHLHe39, c-Myc) Cloning Information Cloning method Restriction Enzyme 5′ cloning site BglII? (not destroyed) 3′ cloning site HindIII (not destroyed) 5′ sequencing primer H1 (Common Sequencing Primers) Resource Information tanner he\u0027s all thatWebUpon transient transfection with plasmid-encoding shRNA, it was found that expression of c-Myc decreased in shRNA-transfected cells, and the downregulation of c-Myc inhibited … tanner health care roanoke alWeb22 nov. 2024 · shRNA-mediated PPARα knockdown in human glioma stem cells reduces in vitro proliferation and inhibits orthotopic xenograft tumour growth. Harry R Haynes, ... In addition, PPARα KD resulted in significant downregulation of the stem cell factors c-Myc, nestin and SOX2. tanner health care for kidsWebFigure 1 The expression of MYC or PLK1, and basal autophagy level in osteosarcoma cells. (A and B) Total RNA were prepared, and subjected to qPCR.ACTB was used as an internal reference. (C and D) Whole cell lysates were prepared, and subjected to Western blot analysis using indicated antibodies.ACTB was used as a loading control. Treatment of 5 … tanner health system employee email