Phospho smad

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August undefined, 2024

WebThe Phospho-SMAD2 (Ser465/4267) assay measures SMAD2 when phosphorylated at Ser465/4267. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. ... HTRF assays facilitate investigation of the TGFB/SMAD/a-SMA signaling axis in liver fibrosis WebAnti-phospho Smad1/Smad5/Smad8 (Ser463/465) AB3848-I Sigma-Aldrich Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Download Zoom Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Antibody, Cat. No. AB3848-I, is a highly specific rabbit polyclonal antibody that targets Smad1//5/8 phosphorylation and has been tested in Immunohistochemi More>>

Phospho-SMAD2 cellular HTRF kit Cisbio - PerkinElmer

WebThis study aimed to elucidate how HCV infection affected hepatic fibro-carcinogenesis, particularly via phospho-Smad signaling. Methods: We first studied phospho-Smad2/3 positivity of 100 patients in different stages of HCV-related chronic liver disease. To examine changes in phospho-Smad2/3 after HCV clearance, we analyzed 32 paired liver ... WebAt 7 days after ischemia, astrocytes that showed strong immunoreactivity for Smad1/5/9 were frequently observed in the CA1 region. Additionally, there was an increase in phosphorylated Smad1/5/9 (phospho-Smad1/5/9) -immunopositive astrocytes in the CA1 region 7 days after ischemia. flower bud clip art

Phospho-SMAD1/SMAD5/SMAD9 (Ser463, Ser465) …

WebNational Center for Biotechnology Information WebRLU = relative light units; p-SMAD2 = phospho-SMAD family member 2. Download Figure Download Powerpoint. Our data suggest that sustained Smad2 phosphorylation in response to TGF-β is dependent on Smad3 activity and is required for myofibroblast differentiation. While acknowledging the role of a canonical immediate Smad2/3-dependent gene ... WebSmad3 (phospho S423 + S425) was labelled with purified ab52903 at a dilution of 1/100 dilution, while Smad3 was labelled with ab207447 at a … greek name for thief

SMAD (protein) - Wikipedia

WebPhospho-Smad2 (Ser465/467) (138D4) Rabbit mAb detects endogenous levels of Smad2 only when dually phosphorylated at serines 465 and 467, and may detect Smad3 … WebThe phospho-SMAD1 (Ser463/465) kit enables the cell-based quantitative detection of SMAD1 phosphorylated at Ser463/465, as a readout of the BMP/SMAD pathway. ... BMP/SMAD signaling pathway plays a key role in embryogenesis, development, adult tissue homeostasis and immune responses. It is mediated by heterotetrameric complexes of … flower bud differentiation stageWebFeb 2, 2024 · We detected both phospho-SMAD3 and phospho-SMAD2 in AR-negative PC3 cells in the absence of TGF-β treatment but those levels significantly increased after TGF-β treatment (Figure 3A). By contrast, phospho-SMAD3 and phospho-SMAD2 were undetectable in AR-positive Rv1 and C4-2 cells, regardless of TGF-β treatment (Figure 3A … flower bud clove thyme plant

"WebAnti-phospho Smad1/Smad5/Smad8 (Ser463/465) AB3848-I Sigma-Aldrich Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Download Zoom Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Antibody, Cat. No. AB3848-I, is a highly specific rabbit polyclonal antibody that targets Smad1//5/8 phosphorylation and has been tested in Immunohistochemi More>> " - Phospho smad

Phospho smad

WebAnti-Smad2 (phospho S255) antibody [EPR2856 (N)] See all Smad2 primary antibodies Description Rabbit monoclonal [EPR2856 (N)] to Smad2 (phospho S255) Host species Rabbit Tested applications Suitable for: Dot blot, IHC-P, IP, WB more details Species reactivity Reacts with: Mouse, Rat, Human Immunogen Synthetic peptide. WebKey features and details Rabbit polyclonal to Smad1 (phospho S187) Suitable for: WB, IHC-P Reacts with: Mouse, Human Isotype: IgG You may also be interested in Primary Anti-Smad2 (phospho S255) antibody …

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WebIn response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. WebPhospho-Smad4 (T277) ELISA Kit (ab279931) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Smad4 protein in your experimental model system, you can verify pathway activation in your cell lysates.

WebZoom Anti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP … WebPhospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb-Phospho-specific Antibodies - ABclonal Log in / Register Product Type Antibodies Primary Antibodies Secondary Antibodies Tag and Loading Control Antibodies Antibody Duos matched antibody pairs Molecular Biology Reagents PCR qPCR Reverse Transcription Ligase Nucleic Acid …

WebPhospho-SMAD1/SMAD5 (Ser463, Ser465) Antibody (700047) in ICC/IF Immunofluorescence analysis of SMAD1/5 (pS463/465) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 … WebSMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by …

WebThe phospho-SMAD3 (Ser423/425) kit enables the cell-based quantitative detection of SMAD3 phosphorylated on Ser423/425, as a readout of the TGFb pathway. ... Overactivation of TGF-ß/SMAD signaling in hepatocytes and HSCs promotes liver fibrosis. Human LX-2* and HepG2 lines were plated (50,000 and 200,000 cells/well respectively) and cultured ...

WebImmunohistochemistry was performed to determine the expression of Smad transducer proteins in NFPAs. Ki-67 was evaluated as an indicator of the proliferative activity of NFPAs. ... Protein levels of Smad3 and phospho-Smad3 (p-Smad3) were significantly lower in patients with invasive NFPAs than in patients with noninvasive NFPAs (P<0.05 and P<0. ... flower budding teaWebSecreted phosphoprotein 24 kD (Spp24) inhibits the growth of human osteosarcoma through the BMP-2/Smad signaling pathway. View PDF In Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society on 8 … flower bud gifWebThis antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested. ... Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis ... flower budget for a weddingWebPhospho-Smad2 (S245/250/255) ELISA Kit (ab279929) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By … greek name for thornWebFig. S1. Endogenous BMP activity in the gut and dorsal mesentery during looping. ( A) Phospho-Smad staining of the dorsal mesentery from E6 through E20. (Scale bar: 100 µm.) ( B) In situ hybridization for bmp2 in the dorsal mesentery and gut tube from E6 through E20. [Scale bar (E6–E10) and (E12–E20): 100 µm.] flower bud iconWebThe Phospho-Smad5 (Ser463,Ser465) Antibody has been validated for the following applications: Western Blot. Western Blot analysis of Phospho-Smad5 (Ser463,Ser465) performed on various cell lines using a Phospho-Smad5 (Ser463,Ser465) monoclonal antibody (Product # MA5-35867) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG … greek name for yahwehWebSMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic … greek name for thomas