Psichecktm-2 vector
WebThen, 100 ng psiCHECK‑2 luciferase reporter plasmids containing WT and M uT 3'-uTr of Bax were co-transfected into B nlcl 2 cells with mi r-214 mimic (100 nM) or mimic control (100 nM) for 48 h using ipofectaminel®2000 (Invitrogen; Thermo Fisher Scientific, Inc.). WebFrom the psiCHECK-2 vector (Promega, Cat. No. C8021), a 231-nucleotide (nt) region was selected from the firefly luciferase gene sequence available for (5) Tumor elimination by …
Psichecktm-2 vector
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WebpsiCHECKTM-2 dual-luciferase reporter plasmid (Promega, cat. no. E1910) to produce psiCHECK-WT-NLRP3 and the mutant vector psiCHECK-MT-NLRP3. Then, the two … Web2.2 测序及序列对比分析 含有hsa-miR-181b结合位点及缺失hsa-miR-181b结合位点的荧光素酶报告载体psiCHECK2-TNF和psiCHECK2-TNF-mut测序结果(图3、图4)。 可见TNF的目标序列和突变序列均成功地克隆入双萤光报告载体psiCHECK2，可用于后续实验。
WebpsiCHECK™-2 Vector Part Numbers: C8021 Vector for Optimizing RNA Interference Assays Monitor changes in expression of a target gene fused to a reporter gene; decreases in luciferase activity correlate with RNAi … WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed by miR-29a, b (except for the 7774–7781 of Col4a3 gene). The inhibitory efficiency of miR-29a, b on these seven sites was between 27% and 57%.
WebNov 17, 2024 · (b) The Pax7 3’UTR with wild-type (wt) or mutant type (mut) were inserted into psiCHECK™-2 vectors and cotransfected with miR-204 mimic or negative control into HeLa cells, respectively. . (c – e) The expression of miR-204 (c), Pax7 (d), and cell division cycle genes ( CCNB (Cyclin B), CCND (Cyclin D), CCNE (Cyclin E), CDK4 (Cyclin-dependent … WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target …
WebNov 18, 2015 · Downregulation of OSBP Expression by miR-124. We used the online microRNA prediction program TargetScan to investigate the potential targets of miR-124 and found four conserved miR-124 target sites in the 3′UTR of OSBP (Fig. 1a).To test whether OSBP was the direct target of miR-124, the 3′UTR of OSBP was cloned into a dual …
Web鸡IGFBP2基因3′UTR区1196C_A单核苷酸多态性的功能性鉴定及分析.pdf sebago long lake music festival youtubeWebAug 1, 2024 · The binding site of miR-509-5p to the TRAF6 3'-UTR region and identification of psiCHECKTM-2 Vector double digests. (A)The binding site of miR-509-5p to the TRAF6 3'-UTR region. Note: The underlined area indicates the target gene mutation site. (B) PCR analysis of recombinant bacteria liquid. sebago litesides leather slip onsWebFeb 8, 2024 · PsiCHECKTM-2 vector without inserted gene was used as negative control (NC) plasmid. The inserted sequences were listed in Table. 1. Following the procedures provided by Ribobio and Promega, A7r5 cells were co-transfected with PsiCHECKTM-2 vector (WT/MUT/NC) and oligonucleotides (miR-137 mimic or mimic control) for 48 hours. pulse physioWebMar 10, 2024 · In order to do a vector search, our input text needs to be converted to an embedding as well. The second parameter to this function “k” is the number of objects we want to return that is closest to our input. We want to find the closest vectors to our input vector so we chain the .with_near_vector() function. sebago leather shoeWebpsiCHECK™-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in … sebago leather lacesWebif the psiCHECK™-2 Vector could report a known miR-21 target (miR-21 mismatch; 5). Complementary oligonucleotides containing the exact miR-21 sequence or a mismatched miR-21 target (5) were generated with flanking partial SgfI and PmeI restriction sites (Table 2). A restriction site not found in the psiCHECK™-2 Vector was also included to pulse photonicsWebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … sebago litesides two eye