Thermo scientific revertaid
WebbThe world leader in serving science Rev. 2A Page 1 of 1 CERTIFICATE OF ANALYSIS K1621 RevertAid 1st cDNA Synth Kit ... 91253674 0.03 mL RiboLock RI 91243230 0.03 mL RevertAid RT 91259221 0.05 mL dNTP Mix 10mM 91218125 0.02 mL Control GAPDH RNA 91211234 0.02 mL Forward Control Primer 91259226 ... Manufactured for Thermo … WebbThermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme …
Thermo scientific revertaid
Did you know?
WebbRevertAid Reverse Transcriptase (Thermo Scientific, USA) on a 7900 HT Fast Real-Time PCR System (Applied Biosystems). RT-qPCR was performed in a 20-µL reaction volume using 1-µL cDNA with SYBR Green Mix (Thermo Scientific, USA) in an ABI 7500 sequence detection sys-tem (Applied Biosystems, USA). GAPDH was used as in-ternal control. WebbRevertAid Reverse Transcriptase (200 U/µL)Thermo FisherCatalog #EP0442 Oligo(dT)18 PrimerThermo FisherCatalog #SO131 BEFORE START INSTRUCTIONS Mix and briefly …
WebbThermo Scientific™ RevertAid RT Reverse Transcription Kit. A complete system for efficient synthesis of first strand cDNA from RNA templates. Brand: Thermo Scientific™ … WebbThermo Scientific RevertAid First Strand cDNA Synthesis Kit with DNase I is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit contains …
WebbThermo Scientific™ RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. The enzyme possesses RNA-dependent and DNA dependent … WebbRevertAid H Minus Reverse Transcriptase, 200 U/µL Analysis 10000 U 5 5X Reaction Buffer 5 1 mL 1 mL Store at -20 °C www.thermofisher.com For Research Use Only. Not for use in diagnostic procedures. Description Thermo Scientific™ RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. The
WebbRevertAid Reverse Transcriptase (200 U/µL)Thermo FisherCatalog #EP0442 Oligo(dT)18 PrimerThermo FisherCatalog #SO131 BEFORE START INSTRUCTIONS Mix and briefly centrifuge all reagents after thawing, keep on ice. 1 Add reaction components into sterile, nuclease-free tube on ice in the indicated order:
Webb25 juni 2024 · 产品说明 RevertAid™第一链 cDNA 合成试剂盒以 mRNA 或者总 RNA 为模板，高效合成第一链 cDNA 。 本 试剂盒使用 RevertAid™ M-MuLV 反转录酶，它的 RNA 酶 H 的活性与AMV 反转录酶相比较低。 该反 转录酶可 耐受 42-50°C 温度，合成的 cDNA 片段长度达 13kb。 试剂盒中含有 RiboLock™ 重组 RNA 酶抑制剂，防止 RNA 降解，可耐受 … rabbit end of lifeWebbRNA extraction was repeated until all RNA samples had an A260/A280 ratio between 1.8 and 2.0. cDNA was synthesized using RevertAid RT Reverse Transcription kit (Thermo Fisher Scientific, Inc.) under the following conditions: 25°C for 5 min, 55°C for 30 min and 75°C for 10 min. sh monsterarts lineupWebbRevertAid Premium Reverse Transcriptase, 200 U/µL 10000 U 4 10000 U 5X RT Buffer 2 1 mL4 Store at -20 °C www.thermofisher.com For Research Use Only. Not for use in diagnostic procedures. Description Thermo Scientific™ RevertAid™ Premium Reverse Transcriptase (RT) is the first reverse transcription rabbit englishWebbThermo Scientific RevertAid RT Tool is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), whose has lower RNase H activity paralleled to AMV annul transcriptase. The enzyme cares activity at 42-50°C and is suited for chemical by cDNA … sh monsterarts godzilla websiteWebb23 aug. 2024 · DESCRIPTION Template RNA PROTOCOLS The Thermo Scientific™ RevertAid™ First Strand cDNA Total cellular RNA isolated by standard methods is I. First Strand cDNA Synthesis Synthesis Kit is a complete system for efficient synthesis suitable for use with the kit. sh monsterarts king ghidorah 2019 ebayWebb20 okt. 2024 · For cDNA synthesis, 5 μg of total RNA was reverse-transcribed in a final volume of 20 μL containing 1 mM dNTPs, 5 μM oligo(dT) 18, 1 unit of an RNase inhibitor, and 10 units of RevertAid M-MuLV reverse transcriptase (Thermo Scientific, Vilnius, Lithuania) for 30 min, followed by incubation for 60 min at 42 °C. sh monsterarts final wars godzillaWebbThermo Scientific RevertAid第一链cDNA合成试剂盒包含了足够进行100次x20 µL反应的试剂。本品是以RNA为模板高效合成第一链cDNA的完整系统，可合成长达13kb的cDNA。 … sh monsterarts heisei godzilla