Transscript® one-step gdna removal

Author: defi

August undefined, 2024

WebApr 13, 2024 · Total RNA was extracted from 200 mg of tuberous roots using a RNAprep Pure Plant Plus Kit (Tiangen, Beijing, China). One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen, Beijing, China) were used for genomic DNA digestion and first-strand cDNA synthesis. Reverse transcription was performed according to the … WebIt is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.

链格孢侵染对甜瓜病程相关蛋白活性及基因表达的影响_参考网

http://www.transgenbiotech.com/rt_pcr/transscript_all_in_one_first_strand_cdna_synthesis_for_qpcr.html WebApr 5, 2024 · 附图说明 [0047] 图1是本发明的一个较佳实施例1的mswrky41基因的pcr ... 提取紫花苜蓿 ‘ wl525’叶片总rna，用transscript one-step gdna removal and cdna synthesis supermix(购自全式金)进行反转录，合成cdna ... maytag 5000 series water level switch

TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix

Web结果表明:损伤接种A.alternata后,伽师瓜和86-1甜瓜均在15 d发病,贮藏过程中伽师瓜果实的发病率及病斑大小均低于86-1甜瓜;甜瓜应答侵染过程中,几丁质酶和β-1,3-葡聚糖酶活力呈现先升高后下降的趋势,分别于21 d,18 d达到酶活高峰,且接种伽师瓜酶活力显著高于86-1甜瓜(p0.05),伽师瓜通过增强病程相关酶活力 ... Webtham, MA, USA). All the RNA samples with A260/A280 ratios = 1.9–2.1 and A260/A230 ratios > 2.0 were used for further study. Then RNA (1 μg) was reversely transcribed into cDNA using a TransScript One-Step gDNA Removal and cDNA Synthesis SuperMIX (Trans-Gen Biotech, Beijing, China) following the manufacturer’s instructions. The … WebTotal RNA was extracted from PBMCs and used to synthesize the cDNA strand using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix ... Rodriguez P, Bonte E, et al. Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. P Natl Acad Sci USA. 2003;100(13) ... maytag 5000 series with steam manual

TransGen Biotech社製品一覧と参考価格 -1- 株式会社バイオロジカ

Transscript One-step Gdna Removal And Cdna Synthesis Kit

http://pakbs.org/pjbot/papers/1681373812.pdf WebSep 12, 2024 · Total RNA was isolated from berry slices using the RNAprep Pure polysaccharide polyphenol plant RNA extraction kit (Tiangen Biotech Co., Ltd., China) as previously reported (Liu et al. 2024b). cDNA for quantitative real-time PCR (RT-qPCR) analysis was obtained using the TransScript® One-Step gDNA Removal and cDNA … maytag 5000 series with steam troubleshootingWebAfter cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85oC for 5 seconds. Highlights • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination. • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is … maytag 5000 series with steam parts

"WebOct 15, 2024 · Total RNA (2.5 μg) was reverse-transcribed to cDNA using TransScript one-step gDNA removal and cDNA synthesis super-mix kit at 42 °C for 15 min following the manufacturer's instructions. The cDNA was diluted 1:10 with nuclease-free water and stored at −80 °C. 2.5. Quantitative real-time PCR analysis " - Transscript® one-step gdna removal

Transscript® one-step gdna removal

TransScript® Uni One-Step gDNA Removal and cDNA Synthesis …

WebUnique genomic DNA remover is combined with TransScript ® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis at 42-55°C. After cDNA synthesis, gDNA remover and TransScript ® II RT/RI Enzyme Mix are inactivated by heating at 85 °C for 5 seconds. • Simultaneous genomic DNA removal … WebAfter the debris was removed, the cells were cultured in serum-free medium for 24 h. Finally, ... Total RNA was reverse-transcribed into complementary DNAs using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech, China). Then, the qRT-PCR was carried out using TransStart TipTop Green qPCR SuperMix ...

Did you know?

WebAfter cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR. • … WebOct 29, 2024 · TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal) Cat. No. AT341 Storage: at -20°C for two years Description …

Web医学实验论文11篇：生物化学检验实验教学改革袁提高学生创新能力等.doc

WebSince a genetics altered print, transgenic soybean occupies an largest global scale with its food, nutritional, industrial, also pharmacological uses.Efficient transformation is a essential factor fork the enhancements of genetically modified soybean. At present, the Agrobacterium-mediated means is primarily used for soybean transformation, but … WebIt is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.

WebThe TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix combines the unique genomic DNA remover with TransScript® First-Strand cDNA Synthesis …

WebApr 13, 2024 · The genes expressed as 0 in all the samples were removed. We took the following steps to screen and identify the lncRNAs: (1) Transcript length filtering, ... Shanghai, China) and reverse transcribed with TransScript®One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen, Beijing, China). maytag 5000 series with steam hookupWebThe kit provides all the necessary reagents for reverse transcription reaction (including TransScript® Uni RT, RNase Inhibitor, Anchored Oligo (dT) 20 Primer, Random Primer … maytag 5000 washer door hingeWeb1. 3 试剂 Ⅱ型胶原酶，Sigma；胰蛋白酶，Ameresco；DMEM／F12混合培养基，Hyclone；胎牛血清（FBS），Gibco（特级）；PBS平衡盐溶液，Sigma；5-溴脱氧尿嘧啶（Brd-u），Sigma；Trizol，Sigma；TransStart Top Green qPCR SuperMix、TransScript®One-Step gDNA Removal and cDNA Synthesis SuperMix均来自于北京全 … maytag 5000 series with steam priceWebwas produced using TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech Beijing Co., LTD). PCR system and procedure are the same as the above, except that the template is cDNA. We examined the PCR products using gel electrophoresis and ligated it into pEASY®-Blunt Zero maytag 5000 washer not drainingWebOct 19, 2015 · 1）α-壳聚糖和β-壳聚糖抑制新城疫病毒的最佳分子量分别为4513Da和5743Da；2）β-壳聚糖的抗新城疫病毒活性 ... ；反转录试剂盒（TransScript One-Step gDNA Removal cDNASynthesis SuperMix，北京全式金生物技术有限公司）；摘要 15 荧光定量试剂盒（TransStart Top Green qRT ... maytag 5000 series with steam electric dryerWebTransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix 50 rxns×20 μl体系 ... 本产品以 RNA 为模板，在同一反应管内，用 TransScript ... 反应结束后，只需在 85℃ … maytag 5000 series with steam dryer for saleWebDec 24, 2024 · MiRNAs are usually produced through a multi-step process, starting from the nucleus and then entering the cytoplasm, consisting of three main ... and TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit (AT311-02, TransGen Biotech, Beijing), respectively. The miScript SYBR Green PCR Kit (Qiagen, Hilden, Germany ... maytag 5000 washer parts